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hek 293ebna cells  (ATCC)


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    Structured Review

    ATCC hek 293ebna cells
    Hek 293ebna Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek+293ebna+cells/293+c18/pmc12744402-104-8-10
    Average 95 stars, based on 202 article reviews
    hek 293ebna cells - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progression.
    Article Snippet: To generate B16-F10 and YUMM5.2 melanoma variants stably expressing nuclear green fluorescent protein (GFP), we fused a nuclear localization signal with GFP in the MSCV-N-Flag-HA-GFP vector (Addgene, Watertown, MA, plasmid #41034, RRID:Addgene_41034). .. This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID:CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral packaging plasmids [11], GagPolS and pN8e-VSV-G, according to the manufacturer’s protocol. ..

    Article Title: Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progression
    Article Snippet: To generate B16-F10 and YUMM5.2 melanoma variants stably expressing nuclear green fluorescent protein (GFP), we fused a nuclear localization signal with GFP in the MSCV-N-Flag-HA-GFP vector (Addgene, Watertown, MA, plasmid #41034, RRID: Addgene_41034). .. This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID: CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral packaging plasmids [ ], GagPolDS and pN8e-VSV-G, according to the manufacturer’s protocol. ..

    Retroviral:

    Article Title: Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progression.
    Article Snippet: To generate B16-F10 and YUMM5.2 melanoma variants stably expressing nuclear green fluorescent protein (GFP), we fused a nuclear localization signal with GFP in the MSCV-N-Flag-HA-GFP vector (Addgene, Watertown, MA, plasmid #41034, RRID:Addgene_41034). .. This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID:CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral packaging plasmids [11], GagPolS and pN8e-VSV-G, according to the manufacturer’s protocol. ..

    Article Title: Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progression
    Article Snippet: To generate B16-F10 and YUMM5.2 melanoma variants stably expressing nuclear green fluorescent protein (GFP), we fused a nuclear localization signal with GFP in the MSCV-N-Flag-HA-GFP vector (Addgene, Watertown, MA, plasmid #41034, RRID: Addgene_41034). .. This vector was packaged into retroviral particles via HEK-293EBNA cells (ATCC, Cat# CRL-10852, RRID: CVCL_6974) co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with the viral packaging plasmids [ ], GagPolDS and pN8e-VSV-G, according to the manufacturer’s protocol. ..

    Generated:

    Article Title: Targeting ITGB2-Driven Tumor Cell-Intrinsic Signaling Inhibits Melanoma Progression
    Article Snippet: Establishment of melanoma lines stably expressing nuclear GFP Nuclear GFP-expressing stable variants of B16-F10 and YUMM5.2 melanoma cells were created by inserting a nuclear localization sequence into the GFP coding region of the MSCV-N-Flag-HA-GFP plasmid (Addgene #41034, RRID: Addgene_41034). .. Retroviruses were generated by transfecting HEK-293EBNA cells (ATCC CRL-10852, RRID: CVCL_6974) with this construct together with packaging plasmids GagPolDS and pN8eVSV-G using Lipofectamine 2000 (Thermo Fisher Scientific) [11]. ..

    Construct:

    Article Title: Targeting ITGB2-Driven Tumor Cell-Intrinsic Signaling Inhibits Melanoma Progression
    Article Snippet: Establishment of melanoma lines stably expressing nuclear GFP Nuclear GFP-expressing stable variants of B16-F10 and YUMM5.2 melanoma cells were created by inserting a nuclear localization sequence into the GFP coding region of the MSCV-N-Flag-HA-GFP plasmid (Addgene #41034, RRID: Addgene_41034). .. Retroviruses were generated by transfecting HEK-293EBNA cells (ATCC CRL-10852, RRID: CVCL_6974) with this construct together with packaging plasmids GagPolDS and pN8eVSV-G using Lipofectamine 2000 (Thermo Fisher Scientific) [11]. ..



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